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Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents.

Haile, Simon; Nikiforuk, Aidan M; Pandoh, Pawan K; Twa, David D W; Smailus, Duane E; Nguyen, Jason; Pleasance, Stephen; Wong, Angus; Zhao, Yongjun; Eisler, Diane; Moksa, Michelle; Cao, Qi; Wong, Marcus; Su, Edmund; Krzywinski, Martin; Nelson, Jessica; Mungall, Andrew J; Tsang, Frankie; Prentice, Leah M; Jassem, Agatha; Manges, Amee R; Jones, Steven J M; Coope, Robin J; Prystajecky, Natalie; Marra, Marco A; Krajden, Mel; Hirst, Martin.

J Virol Methods ; 299: 114339, 2022 01.

Article in English | MEDLINE | ID: covidwho-1472084

ABSTRACT

The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

Subject(s)

COVID-19 , Nucleic Acids , COVID-19 Testing , Humans , Indicators and Reagents , Magnetic Phenomena , Pandemics , RNA, Viral/genetics , SARS-CoV-2 , Sensitivity and Specificity

ABSTRACT

Subject(s)

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